dn/dc and UV Extinction Determination
ASTRA has several different methods for determining the dn/dc or UV extinction coefficients for your sample:
Direct measurement
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Inject aliquots of known concentration into an Optilab or UV detector. ASTRA provides experiment templates for determining dn/dc or UV extinction coefficients. Define baselines, set peak regions with concentrations, then view the corresponding analysis procedure to determine dn/dc or UV extinction. |
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Online measurement
If the assumption can be made that 100% of the injected mass reaches the Optilab or UV detector, it is possible to calculate the dn/dc or UV extinction coefficient online.
Alternately, if an Optilab and UV detector are used in combination, and the dn/dc value is known for the sample, ASTRA can calculate a UV extinction coefficient for the sample.
RI Calibration
If a third-party RI detector is being used, ASTRA provides an experiment template to calculate the RI calibration constant for the analog input signal.
